PEMBUATAN BIOETANOL DENGAN TEKNI IMOBILISASI SEL Saccharomyces cerevisiae DARI LIMBAH KERTAS HVS
Abstract
Paper production in Indonesia based on the Center for Pulp and Paper (BBPK) reaches 12.98 million tons/year. Paper that has been used and thrown away can cause problems for the environment. The cellulose content in paper is very high so that it can be used as a raw material for bioethanol. The fermentation technique used is modern biotechnology, namely the cell immobilization method. This method uses an inert material that is not soluble in the substrate. The purpose of this study is to determine the effects of the concentration of immobilized saccharomyces cerevisiae cells on bioethanol levels to increase the levels of bioethanol produced. Paper was hydrolyzed with 2N HCl to break down cellulose into glucose which would be used as a substrate. The research variable used was the concentrations of immobilized saccharomyces cerevisiae cells, namely 0, 5, 10, 15, 20, and 25% (w/w) in substrate. Fermentation was carried out in a batch system for 4 days. Immobilization of saccharomyces cerevisiae cells was made using 10% Na-Alginate and 7% CaCl2. Ethanol concentration was analyzed using gas chromatography and cell number analysis using gravimetry. The results showed the maximum concentration of bioethanol at a concentration of 15% cell immobilization. Where the concentration of bioethanol was 8.1876% and the number of cells was 1.84 g cells/L
Keywords: bioethanol, fermentation, immobillization, paper, saccharomyces cerevisiae
Keywords
References
Apriyanti, Shella Nenik. (2018). Pengaruh Jenis Asam, Konsentrasi Asam Dan Waktu Hidrolisis Asam Kertas HVS Bekas Pada Proses Fermentasi. Universitas Muhammadiyah Surakarta
Asngad, A,. dan Suparti. 2009. Lama Fermentasi dan Dosis Ragi yang Berbeda pada Fermentasi Gaplek Ketela Pohon Varietas Mukibat terhadap Kadar Glukosa dan Bioetanol. Jurnal Penelitian Sains & Teknologi, Vol. 10, No. 1, 2009: 1 – 9. Universitas Muhammadiyah. Surakarta.
Awaltanova, E., Bahri, S., & Chairul. (2015). Fermentasi Nira Nipah Menjadi Bioetanol Menggunakan Teknik Imobilisasi Sel Saccharomyces cerevisiae. JOM FTEKNIK, 2, 1–7
Bagus, W, G., Buda, Ketut., & Yoga, I Made. (2011). Pengaruh perlakuan delignifikasi dengan Larutan NaOH dan Konsentrasi Substrat Jerami Padi terhadap Produksi Enzim Selulase dari Aspergillus niger Nrrl A-li, 264. Bali: Universitas Udayana
Chairul., & Yenti, Silvia Reni. (2013). Pembuatan Bioetanol dari Nira Nipah Menggunakan Saccharomyces cerevisiae. Jurnal Teknobiologi, IV (2), 105-108
Choirin, M., Jayus., & Sony, S. (2013). Pengaruh ketersediaan oksigen pada produksi epiglukan oleh Epicoccum nigrum menggunakan media molase. Jurnal Agrointek,7.
Kurniawan, R., Juhanda, S., Septiyanti, M., & Resgiaty, Y.(2012). Produksi Etanol Secara Continue dengan Sel Tertambat Menggunakan Bioreactor Tower Fluidized Bed. Prosiding Seminar Nasional Teknik Kimia Kejuangan 2012. ISSN: 1693-4393.
Marlinda., Ramli., & Ardis. (2019). Pengaruh Konsentrasi Imobilisasi Sel Saccharomyces Cerevisiae Pada Pembuatan Bioetanol Dari Nira Nipah. Prosiding Seminar Nasional, 135–139.
Nabil, Muhammad. (2020). Pengaruh Jumlah Ragi Roti (Saccharomyces cerevisiae) dan Lama Fermentasi Kertas HVS Bekas Pakai Terhadap Kadar Etanol. Malang: Universitas Islam Maulana Malik Ibrahim.
DOI: https://doi.org/10.46964/jimsi.v1i2.865
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 JURNAL TEKNIK KIMIA VOKASIONAL (JIMSI)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Editor Jurnal Teknik Kimia Vokasional (JIMSI) address :
ISSN 2775-9083 (Print) | ISSN 2775-9075 (Online)
Department Of Chemical Engineering, Politeknik Negeri Samarinda
Jl. Dr. Ciptomangunkusumo Kampus Gunung Panjang Samarinda 75131
Phone: Zainal Arifin (+62 857 500 60 400)
E-Mail: jimsi@polnes.ac.id
Jurnal Teknik Kimia Vokasional (JIMSI) by http://e-journal.polnes.ac.id/index.php/jimsi is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.